91禁在线看 I 最近最好的2019中文 I 国产一级二级三级 I 亚洲成人av在线播放 I 91视频麻豆 I 国产999精品 I yy6080午夜 I 免费看黄色小视频 I 人妻av一区二区 I 美女羞羞动态图 I 免费日皮视频 I 黄色免费网页 I 动漫3d精品一区二区三区乱码 I 美女扒开粉嫩尿口 I 日韩av一区二区在线播放 I 欧美激情18p I 一级色网站 I 久久久男人的天堂 I 国产精品香蕉国产 I 狠狠插影院 I 亚洲成人一级 I 在线免费观看日韩视频 I 免费公开在线视频 I 午夜寂寞自拍 I 日韩国产91 I 久久不卡 I 日本激情一区 I 夜色福利

Iso-Insulin EIA 同工胰島素檢測(cè)試劑盒介紹：

Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of insulin in human serum or plasma.

Iso-Insulin EIA 同工胰島素檢測(cè)試劑盒Summary and explanation of the test

Insulin is the principal hormone responsible for the control of glucose metabolism.It is synthezised in the ?-cells of the islets of Langerhans as the precursor,proinsulin, which is processed to form C-peptide and insulin. Both are secretedin equimolar amounts into the portal circulation. The mature insulin moleculecomprises two polypeptide chains, the A chain and B chain (21 and 30 aminoacids respectively). The two chains are linked together by two inter-chaindisulphide bridges. There is also an intra-chain disulphide bridge in the A chain.?Secretion of insulin is mainly controlled by plasma glucose concentration,and the hormone has a number of important metabolic actions. Its principalfunction is to control the uptake and utilization of glucose in peripheral tissuesvia the glucose transporter. This and other hypoglycaemic activities, such as theinhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by thehyperglycaemic hormones including glucagon, epinephrine (adrenaline), growthhormone and cortisol.?Insulin concentrations are severely reduced in insulin-dependent diabetesmellitus (IDDM) and some other conditions such as hypopituitarism. Insulinlevels are raised in non-insulin-dependent diabetes mellitus (NIDDM), obesity,insulinoma and some endocrine dysfunctions such as Cushing’s syndrome andacromegaly.

Iso-Insulin EIA 同工胰島素檢測(cè)試劑盒Principle of the procedure

Mercodia Iso-Insulin ELISA is a solid phase two-site enzyme immunoassay. It isbased on the direct sandwich technique in which two monoclonal antibodies aredirected against separate antigenic determinants on the insulin molecule. Duringincubation insulin in the sample react with peroxidase-conjugated anti-insulinantibodies and anti-insulin antibodies bound to microtitration well. A simplewashing step removes unbound enzyme labeled antibody. The bound conjugate isdetected by reaction with 3,3’,5,5’-tetramethylbenzidine. The reaction is stoppedby adding acid to give a colorimetric endpoint that is read spectrophotometrically